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PARISS Imaging spectrometer

PARISS® Modular Imaging Spectrometer And Spectrograph

 

The PARISS imaging spectrometer is prism-based for the highest possible spectral sensitivity.

Assemble the perfect system for your application and budget.

PARISS Imaging Spectrometer Modules Include:

Zoom magnificationFrom less than 1x to greater than 40x. Eliminates the need for multiple objectives

Samples and specimens: Designed to work with heterogeneous samples in air, a petri dish, microscope slide, in water or in-situ

Optics and objectives: Accommodates high numerical aperture (NA) long working distance objectives, commercial microscope objectives, camera, and long focal length telescope lenses for remote sensing.

Spectroscopy modes: %reflection, %transmission, absorption fluorescence, luminescence…

Wavelength range: prism-based imaging spectrograph. Operates from  360 – 1,000-nm depending on the spectrum camera and application optics.

Mounts: Can be mounted on a column, microscope C-mount video port, a cart, tripod, or mast

Illumination:  LED brightfield, darkfield, refection, transmission, monochromatic fluorescence excitation

Observed field-of-view: Coaxial side camera records the FOV live.  Great for object targeting and documentation

Sample translation: Computer-controlled translation stage.  Accommodates high- and low-resolution imaging

The PARISS Prism Imaging Spectrometer|Spectrograph Overview

Overview: A modular prism based imaging spectrometer and spectrograph captures low signal- to-noise, spatially resolved spectra, at all wavelengths from 365-nm – 920-nm simultaneously.

When used with a CCD or CMOS camera spectrum detector it becomes an imaging spectrometer. Enables point-to point spectral imaging in a variety of configurations (see Figures 1, 2 and 3).

Design: The PARISS imaging spectrograph and spectrometer uses a prism with curved sides to deliver state of the art light throughput efficiency.  This enables highest sensitivity and very fast acquisition times, even with low signal to noise spectra. 

Zoom magnification is available from less than 1x (de-magnification) to 40x, or higher with microscope objectives.

Applications include:

  • Biological and medical research
  • Solar energy research
  • Detection of toxic algae (cyanobacteria that can produce deadly cyanotoxins) (Figure 4)
  • Detection of microplastics in sediment (Figure 5)
  • Light-emitting devices
  • Photo-luminescent materials
  • Forensic materials
  • Industrial Q.C.

Macro-PARISS “kit:”  Most PARISS modules are available separately.  Our goal is to enable any researcher to mix-and-match and buy only what is needed.  When budgets are squeezed this is a great way to save money.

Upgrade any time as funds become available.

Mounting: PARISS imaging spectrograph can be column mounted on a bench, a tripod, or interface with a microscope video port.

Light collection optics can include a c-mount macro lens, with or without zoom capabilities, a microscope objective or telescope optics.

Spectral object characterization in %reflection, absorption, or luminescence

Spectral cameras: can be user-supplied or select from a range of options available through LightForm.

Software: Written in Python, various options are available including:

  • Basic spectral analysis %Refection, absorption, emission
  • Spectral classification
  • Create spectral libraries
  • Perform spectral recognition (Figure  6)

Go here to compare the spectral properties of prisms vs gratings

Partner companies include, but are not limited to: NavitarPrior Scientific,

Thor Labs, The Imaging SourceEdmund Optics…

PARISS Specifications

  • Weight: 1,250 g (Excluding a camera)
  • Moving parts: None. Optimizes stability and reproducibility. 
  • Dimensions: 210 x 55 x 85 mm
  • Wavelength dispersive element:  The wavelength dispersive element is a prism with optical “power.” Concave and convex surfaces on the front and rear surfaces correct astigmatism, coma, and spherical aberration. (See Figure 2)
  • Spectral range: 365 to ~920 nm or 400 to ~920 nm, depending on choice of camera.  All spectra acquired simultaneously without order sorting filters
  • Light throughput efficiency: Internal transmission ~90% from 450 to ~920 nm.
  • Entrance slit dimensions: Standard 5 mm. by 25 micron, widths of 50 and 100 micron are available in pre-aligned mounting assemblies.
  • Spatial resolution at the sample: Depends on slit width and camera pixel size ~ 0.6 micron by ~0.6 micron with 40x magnification typical.  Nanoparticles may be detected but not resolved
  • Spectral resolution: ~1 nm measured at the full width at half maximum of the 436 nm Hg line, depends on slit width and camera pixel size.
  • Optional calibration standards: Available MIDL wavelength calibration lamp and a “SYLPH”  NIST certified radiometric light source.

Modular Imaging Spectrometer Fundamentals

What is an imaging spectrometer?

An imaging spectrometer consists of an imaging spectrograph plus a CMOS or CCD camera to acquire and quantify spectra. The goal is to identify and map the location of objects or conditions in a heterogeneous field-of-view. Knowing the spectral characteristics of target objects or conditions enables the use of classic spectroscopic methods for identification The key difference between a classic spectrometer and an imaging spectrometer is the ability to deliver spatial, point-to-point imaging

What spectral resolution can I expect from an Imaging spectrometer

Changing bandpass or spectral resolution is not usually a user-adjustable function. It is the entrance slit of the spectrometer that controls the bandpass and spectral resolution of the instrument. For example, the PARISS prism-based imaging spectrometer delivers 1-nm bandpass with a standard slit-width of 25-micron. Supplying the system with a 50-micron slit-width reduces the spectral resolution to 2-nm, but doubles light throughput. It is always best to discuss the needs of an application before purchasing a system.

What do you mean by a “modular” imaging spectrometer?

The PARISS modular imaging spectrometer consists of the PARISS imaging spectrograph plus a wide selection of optics that image the field of view onto the spectrometer. For example, detecting microplastics or skin lesions may be best served with a zoom magnification. A second observed image camera can be mounted to enable precise targeting and full-color documentation. Each module can be purchased separately, and the system can be modified or updated to meet diverse application demands at any time. Starting with a basic system and updating it as funds become available, or applications change, adds flexibility and considerable cost savings to the user.

What is better, a prism or a diffraction grating-based imaging spectrometer?

The effectiveness of an imaging spectrometer is all about sensitivity, and light throughput (signal) is the key. A typical diffraction grating offers about 60% efficiency at only one wavelength. In comparison, a prism offers over 90% efficiency over a very wide wavelength range. Consequently, a prism delivers higher signal-to-noise ratios and shorter acquisition times than a diffraction grating. The PARISS® imaging spectrometer is prism-based and delivers many hundreds of spatially resolved spectra simultaneously, often in milliseconds. What are the spectroscopic modes of operation of the PARISS imaging spectrometer? The PARISS imaging spectrometer performs basic spectroscopy that includes %reflection, absorption, fluorescence, luminescence, and dark-field scatter. .

What software does an imaging spectrometer need?

Unlike a classical spectrometer that acquires one spectrum at a time, imaging spectrometers acquire many hundreds of spectra, each with hundreds of wavelength data points simultaneously. The enormous number of acquired spectra presents a significant challenge to most commercial off-the-shelf spectroscopy software packages. To solve this problem, LightForm provides custom PARISS software, written in PYTHON, to process the many hundreds of spectra acquired simultaneously. The software also controls the operating parameters of the spectral camera and ancillary observed image cameras. Features include spectral acquisition, classification, and the creation of spectral libraries that correlate with target objects. Spectral libraries identify target objects as a function of their spectral characteristics.

Imaging spectrometer types: prism, diffraction grating and electronic filter

Imaging spectrometers are available in three formats: electronic filters that include acousto-optic tunable filters (AOTF), liquid crystal filters (LCD), and interferometers. Filter type imaging spectrometers acquire a fixed field-of-view through a series of bandpass filters sequentially. Prism and diffraction grating imaging spectrometers (wavelength dispersive) acquire an unlimited field-of-view sequentially and all wavelengths simultaneously. Wavelength dispersive instruments tend to be analytical and filter systems relative.

Click here for a performance comparison between
a prism and a diffraction grating

The PARISS imaging spectrometer interfaced with a microscope

Figure 1: The PARISS imaging spectrometer interfaces with the video port of a microscope

Cart-mounted imaging spectrograph for laboratory, clinic or field

Figure  2: Portable, cart-mounted imaging spectrometer for laboratory, clinic or field

PARISS imaging spectrometer tripod mounted for Remote Sensing

Figure 3: Macro Pariss imaging spectrometer for remote spectral imaging in the field

Pond with algal bloom.  Spectrum insert captured with the PARISS imaging spectrometer confirms the presence of toxic cyanobacteria.

Figure 4: Pond with algal bloom.  Spectrum insert captured with the PARISS imaging spectrometer confirms the presence of toxic cyanobacteria.

Spectral image of microplastics

Figure 5: Spectral image of microplastics taken in darkfield reflection

Figure 6:  Python, imaging spectroscopy software for spectral classification and recognition

How PARISS Analytical Hyperspectral Imaging Works

How PARISS Hyperspectral Wavelength Dispersive Imaging Works

Darkfield hyperspectral nanoparticle characterization 

How PARISS hyperspectral imaging microscopy works

PARISS hyperspectral imaging microscopy modes of operation

All PARISS Hyperspectral systems are custom configured to meet the needs of an application.
The above configuration is for
guidance only. Specifications can and do change without notice.

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