Multispectral Imaging (MSI): Defined as a field of view that has been characterized over a Non Contiguous wavelength range. This is typically achieved by using a series of bandpass filters generated electronically, for example a Liquid Crystal Tunable Filter (LCTF), an Acousto-Optic Tunable Filter (AOTF) or an interferometer. Up to 50  non-contiguous Wavelength Data Points (WDP) are acquired sequentially over a FIXED field of view.   Electronic filters precede the front lens of a camera. MSI data is rarely of “spectroscopic” value and cannot be used with mobile or otherwise changing samples. View the animation movie below Hyperspectral Imaging instruments (HSI): Use an imaging spectrometer with either a diffraction grating or prism to acquire many hundreds of WDP contiguous wavelengths simultaneously.  Data is typically of spectroscopic quality.  The technique is often referred to as “Push Broom” Hyperspectral imaging, and was developed to accommodate a fast changing FOV. 
1: Approaches to Spectral Imaging Hardware, Lerner JM, Gat N, Wachman E., Current Protocols in Cytometry 12.20.1-12.20.40, July 2010
Multi-spectral and Hyperspectral Methods Overview
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Copyright © LightForm Inc, 2017
Video illustrates how LCTF, AOTF and interferometers used in multi-spectral imaging system acquire spectral data.
LightForm Inc: Pioneering Analytical Hyperspectral Microscopy Since 1996
Video illustrates how prism and diffraction grating based Hyperspectral microscope systems acquire spectral data.
For a technical performance comparison between Multi- spectral and Hyperspectral instrumental methods go here:
Tech comparison Tech comparison Click to play multispectral imging animation Click to play Hyperspectral imaging animation
Multispectral Imaging (MSI): Defined as a field of view that has been characterized over a Non Contiguous wavelength range. This is typically achieved by using a series of bandpass filters generated electronically, for example a Liquid Crystal Tunable Filter (LCTF), an Acousto-Optic Tunable Filter (AOTF) or an interferometer. Up to 50  non-contiguous Wavelength Data Points (WDP) are acquired sequentially over a FIXED field of view.   Electronic filters precede the front lens of a camera. MSI data is rarely of “spectroscopic” value and cannot be used with mobile or otherwise changing samples. View the animation movie below Hyperspectral Imaging instruments (HSI): Use an imaging spectrometer with either a diffraction grating or prism to acquire many hundreds of WDP contiguous wavelengths simultaneously.  Data is typically of spectroscopic quality.  The technique is often referred to as “Push Broom” Hyperspectral imaging, and was developed to accommodate a fast changing FOV.  
1: Approaches to Spectral Imaging Hardware, Lerner JM, Gat N, Wachman E., Current Protocols in Cytometry 12.20.1-12.20.40, July 2010
Multi-spectral and Hyperspectral Methods Overview
LightForm_Logo
Copyright © LightForm Inc, 2017
Video illustrates how LCTF, AOTF and interferometers used in multi-spectral imaging system acquire spectral data.
LightForm Inc: Pioneering Analytical Hyperspectral Microscopy Since 1996
Video illustrates how prism and diffraction grating based Hyperspectral microscope systems acquire spectral data.
For a technical performance comparison between Multi- spectral and Hyperspectral instrumental methods go here:
Tech comparison Tech comparison Click to play multispectral imging animation Click to play Hyperspectral imaging animation