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PARISS darkfield hyperspectral microscope

Darkfield Hyperspectral Nanoparticle Imaging

 

Darkfield Hyperspectral Nanoparticle Imaging and characterization of biological and industrial materials using hyperspectral microscopy 

Hyperspectral nanoparticle image with correlated spectra

Nanoparticles with different sizes and shapes scatter light with spectral differences.  Each color correlates with a specific “class” of nanoparticle

Darkfield Hyperspectral Nanoparticle Imaging Characterization, And Mapping

Darkfield illumination is made possible by observing objects in a field of view (FOV) in oblique illumination.  This enables nano-scale objects to be observed in scattered light.  Nanoparticles of different sizes and shapes reflect scattered light  with spectral differences.  Note that nanoparticles can be detected and characterized, but often cannot be individually resolved in scattered light

These differences can be quantified and mapped to a FOV using hyperspectral imaging. Spectra are recorded, characterized, inserted into a spectral library and pseudo-colored.  Each of these colors then correlate with a given spectrum, that in turn correlates with a class of nanoparticles.

Custom software optimized to work in low signal to noise (S/N) environment.  Team LightForm realized that traditional hyperspectral imaging software just could not handle the challenges presented by low S/N spectral nanoparticle characterization.

After extensive testing team LightForm developed a family of custom algorithms used extensively in the seismology community.  Based on cross correlation analysis (CCA) these algorithms are not impacted by spectral non-linearity and hold up very well when signals are “buried in noise”.  When used with spectral wave forms “CCA” becomes “SWCCA.”

SWCCA classifies spectra presented by the FOV, generates reference spectral libraries (RSL), and correlates sample spectra with RSL  spectra.  Then the software uses SWCCA to  create spectral topographical maps of the FOV (hyperspectral images.)  (Details)

Calibration and validation:  In order for all researchers and instruments to be able to interchange data LightForm provides tools for wavelength calibration and radiometric correction..

Once a hyperspectral microscope has been “normalized” all instruments  that are wavelength accurate and radiometric will be able to interchange reproduce data.

Oblique illumination enables darkfield illumination

Darkfield Hyperspectral Nanoparticle Characterization Basics

Characterize these nano-scale materials

  • Metallic (Ag, Au, Co, TiO2, Ce oxides…) as nanoparticles
  • Non-metallic nanoparticles (Carbon nanotubes…)
  • 5 to >200 nm particles, depending on material and illumination light source

Darkfield hyperspectral nanoparticle Imaging properties

  • Maps the location and character of nanoparticles in a field of view
  • Non destructive: Benign illumination keeps live cells alive!
  • Work in high or low magnification: 10 to 100X
  • Works in Surface Plasmon Resonance (SPR) to reveal size, shape and proximity

Sample types

  • Live cells, tissue or prepared samples
  • Powders, cosmetics…
  • Paints, ceramics, abrasives
  • Industrial materials
Darkfield hyperspectral image of gold nanoparticles in cells

Darkfield Hyperspectral Image Of Gold Nanoparticles In Cells

Hyperspectral image of gold nanoparticles in live cells revealed in darkfield scatter

Dakfield hyperspectral image of silver nanoparticles

Darkfield hyperspectral Image of silver nanoparticles in cells

Hyperspectral image of silver nanoparticles in prepared cells. Each color represents a specific spectrum
Darkfield hyperspectral scatter intensity versus wavelength

Nanoparticle intensity vs wavelength

A Xenon light source provides the intense blue light required for maximum S/N ratio. 

Xenon provides over 10X the UV/blue wavelengths compared with a halogen lamp

Darkfield hyperspectral image of gold/nickel nanorod

Darkfield hyperspectral image of metals in a nanorod

PARISS records the spectra and location of gold and nickel that make up this complex nanorod

How PARISS Analytical Hyperspectral Imaging Works

How PARISS Hyperspectral Wavelength Dispersive Imaging Works

Darkfield hyperspectral nanoparticle characterization 

How PARISS hyperspectral imaging microscopy works

PARISS hyperspectral imaging microscopy modes of operation

All PARISS Hyperspectral systems are custom configured to meet the needs of an application. The above configuration is for guidance only. Specifications can and do change without notice.