Nanoparticles with different sizes and shapes scatter light with spectral differences. Each color correlates with a specific “class” of nanoparticle
Darkfield hyperspectral nanoparticle imaging characterization, and mapping
Darkfield illumination is made possible by observing objects in a field of view (FOV) in oblique illumination. This enables nano-scale objects to be observed in scattered light. Nanoparticles of different sizes and shapes reflect scattered light with spectral differences. Note that nanoparticles can be detected and characterized, but often cannot be individually resolved in scattered light
These differences can be quantified and mapped to a FOV using hyperspectral imaging. Spectra are recorded, characterized, inserted into a spectral library and pseudo-colored. Each of these colors then correlate with a given spectrum, that in turn correlates with a class of nanoparticles.