LightForm_Logo
Copyright © LightForm Inc, 2017
LightForm Inc: Pioneering Analytical Hyperspectral Microscopy Since 1996
Beads presenting any of eight different fluorophores Tissue section muliplexed with three overlapping fluorophores Spectra of overlapping fluorophores
The PARISS Hyperspectral Fluorescence Imaging System is an accessory That Attaches to the Video Port of a Research Microscope.
Typical Applications for Fluorescence Hyperspectral Imaging   Characterize CFP/YFP FRET: with over 300 wavelength data points! A recent FRET paper included the surprising statement:     Quote: “While the biological implication for such protease activity we observed in some dying cells remains to be explored, these examples demonstrate the power of spectral imaging to monitor FRET. None of the other FRET measurement methods, including sensitized emission, acceptor recovery after photo-bleaching or donor fluorescent lifetime imaging (FLIM), can differentiate such situations.”[1] Segment and delineate: Up to 15 fluorophores or chromophores simultaneously in fluorescence, luminescence, or brightfield Fluorescent Nano-materials: Quantum Dots, nanowires, nanorods and nanoparticles OLED: (Organic LED) using auto-fluorescence for defect characterization Spectral "unmixing" algorithm: spectral waveform cross-correlation. Accommodates non-linear mixing

The PARISS® Fluorescence Hyperspectral Imaging

     [1] "Detection of Real-Time Mitochondrial Caspase Activity, in Situ, in Live Cells", Yingpei Zhang, Catherine Haskins, Marisa Lopez-Cruzan, Jianhua Zhang, Victoria Centonze-Frohlich, Brian Herman; Microsc Microanal.; 10:442-8 (Aug 2004)
Tissue section multiplexed with three highly overlapping fluorophores
5 micron beads multiplexed with 8 highly overlapping fluorophores
Spectra of eight highly overlapping fluorophores found in beads
LightForm_Logo
Copyright © LightForm Inc, 2017
LightForm Inc: Pioneering Analytical Hyperspectral Microscopy Since 1996
Beads presenting any of eight different fluorophores Tissue section muliplexed with three overlapping fluorophores Spectra of overlapping fluorophores
The PARISS Hyperspectral Fluorescence Imaging System is an accessory That Attaches to the Video Port of a Research Microscope.
Typical Applications for Fluorescence Hyperspectral Imaging   Characterize CFP/YFP FRET: with over 300 wavelength data points! A recent FRET paper included the surprising statement:     Quote: “While the biological implication for such protease activity we observed in some dying cells remains to be explored, these examples demonstrate the power of spectral imaging to monitor FRET. None of the other FRET measurement methods, including sensitized emission, acceptor recovery after photo-bleaching or donor fluorescent lifetime imaging (FLIM), can differentiate such situations.”[1] Segment and delineate: Up to 15 fluorophores or chromophores simultaneously in fluorescence, luminescence, or brightfield Fluorescent Nano-materials: Quantum Dots, nanowires, nanorods and nanoparticles OLED: (Organic LED) using auto-fluorescence for defect characterization Spectral "unmixing" algorithm: spectral waveform cross-correlation. Accommodates non- linear mixing
The PARISS Fluorescence Hyperspectral Imaging
 [1] "Detection of Real-Time Mitochondrial Caspase Activity, in Situ, in Live Cells", Yingpei Zhang, Catherine Haskins, Marisa Lopez-Cruzan, Jianhua Zhang, Victoria Centonze-Frohlich, Brian Herman; Microsc Microanal.; 10:442-8 (Aug 2004)
Tissue section multiplexed with three highly overlapping fluorophores
5 micron beads multiplexed with 8 highly overlapping fluorophores
Spectra of eight highly overlapping fluorophores found in beads